Kit for detecting lung cancer EGFR gene mutation targeted therapy related drug-resistant variation sites

The invention discloses a kit for detecting lung cancer EGFR gene mutation targeted therapy related drug-resistant variation sites. The kit comprises amplimers represented by SEQ ID NO.1 to SEQ ID NO.122 and extension primers represented by SEQ ID NO.123 to SEQ ID NO.183. The kit can detect 60 nucleic acid sites of the following seven relevant genes: EGFR, KRAS, PIK3CA, BRAF, HER2, BIM and NRAS, provides gene analysis data for successful or failed EGFR targeted therapy, and provides basis for subsequent therapy and researches.