FCHO1560−571 peptide, a PKB kinase motif, inhibits tumor progression

Abstract Background Cell division is regulated by protein kinase B (PKB)-mediated FCH domain only 1 (FCHO1) phosphorylation. Methods FCHO1560−571, a synthetic water-soluble peptide, was generated from the PKB substrate motif 560PPRRLRSRKVSC571 found in the human FCHO1 protein. Results In this study, we found that in vitro FCHO1560−571 inhibits cell proliferation via PKB/ERK/SMAD4 pathways in KRAS-mutated A549 lung cancer cells. In addition, FCHO1560−571, at effective doses of 15 and 30 mg/kg, significantly suppressed tumor growth and decreased the size and weight of tumors in A549-xenograft mice. Conclusion These results suggest that the FCHO1560−571 peptide could be a potential therapy for lung cancer.