Sesquiterpenoids from the Whole Plants of Chloranthus holostegius and Their Anti‐inflammatory Activities

Main observation and conclusionFive new sesquiterpenoids (1-5), chloranholides A-E, along with three known compounds (6-8), were isolated from the whole plants of Chloranthus holostegius. Their structures were elucidated using the spectroscopic methods (IR, UV, HR-ESI-MS, 1D and 2D NMR), and their absolute configurations were determined by the X-ray crystallography and ECD calculation. Compounds 1-8 were evaluated for their anti-inflammatory effects on LPS-induced RAW 264.7 macrophage cells. Especially, compound 4 exhibited the most significant anti-inflammatory activity with the secretion levels of TNF-alpha and IL-6 at 15.52% and 5.98%, respectively, better than that of dexamethasone.