Method for detecting toxicity of non-mucus klebsiella pneumoniae

user-5bd69975530c70d56f390249(2020)

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摘要
The invention discloses a method for detecting toxicity of non-mucus klebsiella pneumoniae, and belongs to the technical field of biological detection. The method comprises the following steps: takingan ampR gene sequence in a high-toxicity non-mucus klebsiella pneumoniae genome as a marker, designing a primer by using the specific sequence, and finally completing qualitative detection on the toxicity of the non-mucus klebsiella pneumoniae based on polymerase chain reaction. The method overcomes the defect that: the toxicity of the klebsiella pneumoniae can only be judged by judging whether the klebsiella pneumoniae is mucus or not and the toxicity difference in non-mucus strains cannot be distinguished in qualitative detection methods of the klebsiella pneumoniae in the prior art, so that the application values of the marker and the detection method for detecting the toxicity of the non-mucus klebsiella pneumoniae are guaranteed.
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关键词
Klebsiella pneumoniae,Polymerase chain reaction,Primer (molecular biology),Toxicity,Mucus,Microbiology,Genome,Biology,Gene sequence
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