A New Method for Sequencing the Mitochondrial Genome by Using Long Read Technology.

Sophie Dhorne-Pollet,Nicolas Pollet,Eric Barrey

MITOCHONDRIAL MEDICINE, VOL. 3, 2 EDITION: Manipulating Mitochondria and Disease- Specific Approaches（2021）

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摘要

We describe a protocol to prepare a multiplexed mtDNA library from a blood sample for performing a long read sequencing of the mitochondrial genome. All steps are carefully described to get a high enrichment of mtDNA relative to total DNA extracted from the blood sample. The obtained mutiplexed library allows the production of long sequence mtDNA reads up to 16.5 kbp with a quality enabling variant-calling by using a portable sequencer (MinION, Oxford Nanopore Technologies).

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关键词

Long read sequencing,MinION,Mitochondrial DNA,Multiple displacement amplification,Nuclear DNA depletion

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