PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.
Methods in molecular biology (Clifton, N.J.)(2021)
Abstract
Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for simultaneous isolation of mtDNA and nuclear DNA (nucDNA), and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material, and availability of specific PCR reagents. We also briefly describe factors that affect mtDNA copy number and discuss caveats to its use as a biomarker.
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Key words
Copy number,Mitochondrial DNA,Mitochondrial disease,Mitochondrial toxicity,QPCR,mtDNA,mtDNA depletion
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