Ultrasonic modes to improve the optical clearing of the skin ex vivo

The experimental study of ultrasonic modes was performed for ex vivo skin samples of white Wistar rats. The ultrasound was provided by a Dynatron 125 US system (Dynatronics, USA) after applying an optical clearing agent aqueousalcohol- fructose (20%-30%-50%) solution to the sample surface. Three different ultrasonic modes were used to found the most effective and fastest optical clearing. The optical coherence tomography system, Spectral Radar OCT System OCP930SR 022 (Thorlabs Inc., USA) with a wavelength of 930 nm, was used for obtaining the quantitative data for effectiveness and rate of skin optical clearing. The best mode had a frequency of 3 MHz and a power of 1.5 W in continuous wave mode for 3 minutes.