Enhanced hydrolytic efficiency of an engineered CBM11-glucanase enzyme chimera against barley β-d-glucan extracts
Food Chemistry(2021)
摘要
•- End-to-end fusion of a 1,3–1,4-glucanse with a family 11 carbohydrate-binding module.•- The heterologous chimera has catalytic activity and native-like secondary structure.•- The chimera shows increased catalytic efficiency as compared to the unfused enzyme.•- The chimera shows increased hydrolysis of malted and unmalted crude barley extracts.
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关键词
Family 11 carbohydrate-binding module,GH16 β-1,3-1,4-Glucanase,Chimeric protein,Brewing,Barley β-glucan degradation
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