Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse
STAR protocols(2021)
Abstract
Summary: While isolating immune cells from spleens and lungs is routinely achieved using flow cytometry, it is challenging to isolate viable immune cells from skin. Here, we describe a step-by-step protocol for skin digestion using a murine melanoma model, which is amenable for detection of low abundant immune cell populations including group 2 innate lymphoid cells.
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Key words
Cancer,Cell isolation,Flow Cytometry/Mass Cytometry,Immunology,Model Organisms
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