Additional file 1: Figure S1. of Quercetin-induced miR-200b-3p regulates the mode of self-renewing divisions in pancreatic cancer

Conditioned experiement was performed to confirm whether the effects of quercetin are due to the upregulation of miR-200b-3p, using eight conditions as follows: (1) Control, untreated cells (CO), (2) MiRNA negative control (miR-NC), (3) MiR-200b-3p inhibitor (miR-200b-), (4) MiR-200b-3p mimic (miR-200b+), (5) Quercetin (Q), (6) Quercetin plus miRNA negative control (Q+miR-NC), (7) Quercetin plus miR-200b-3p inhibitor (Q+miR-200b-), (8) Quercetin plus miR-200b-3p mimic (Q+miR-200b+). Pancreatic cancer cell line AsPC1 in the aforementioned treatment groups were evaluated for tumorsphere formation. The resulting tumorspheres were evaluated and thereafter dissociated into single cells and the cell numbers evaluated. The resulting cells were plated with DMEM + FCS + HEPES. After 72h of treatment as listed above, the cells were evaluated for osteogenic and chondrogenic differentiation. (PDF 188 kb)