Analysis of local protein accumulation kinetics by live-cell imaging in yeast systems.

Microscopy-based analysis of protein accumulation at a given subcellular location in real time provides invaluable insights into the function of a protein in a specific process. Here, we describe a detailed protocol for determining protein accumulation kinetics at the division site in the budding yeast and fission yeast . This protocol can be adapted for the analysis of any protein involved in any process as long as the protein is localized to a discrete region of the cell. For complete details on the use and execution of this protocol, please refer to Okada et al. (2021) and Okada et al. (2019).