Chromatin Immunoprecipitation Protocol for Circadian Clock Proteins.

Chromatin immunoprecipitation, or ChIP, is a powerful experimental technique for probing protein-DNA interactions in vivo. This assay can be used to investigate the association of a protein of interest with specific target loci. Alternatively, it can be combined with high-throughput sequencing technology to identify genome-wide binding sites. Here, we describe a ChIP protocol that was optimized for low-abundance transcription factors in Arabidopsis, and provide guidance on how to adapt it for other types of plants and proteins.