Profiling Of Plasma Circulating Mirna In Coronary Heart Disease Patients Detected By Next-Generation Small Rna Sequencing

MiRNA levels in plasma have emerged as potential novel noninvasive biomarkers for some diseases. We aimed to identify a miRNA expression profile in plasma of patients with coronary heart disease. The levels of plasma miRNA from a cohort of 49 patients with coronary heart disease and 13 healthy controls were detected by small RNA deep sequencing, the differentially expressed miRNAs were validated using the reverse transcription real-time quantitative polymerase chain reaction from an additional cohort of 135 hospitalized patients and 140 controls. Five miRNAs were differentially expressed, miR-30c-2-3p and miR-5091 were upregulated and miR-125b-5p, miR501-3p, and miR-31-5p downregulated in plasma of patients with coronary heart disease than in controls. This study identified 5 plasma miRNAs that might play an important role in the pathogenesis of coronary heart disease. The level of miR-30c-2-3p was 132 folds upregulation (P=0.000), it suggests that miR-30c-2-3p may be a potential noninvasive biomarker for the diagnosis of coronary heart disease, however, with further research.