Lineage-Specific Real-Time Reverse Transcription Pcr For Yellow Fever Virus Outbreak Surveillance, Brazil
EMERGING INFECTIOUS DISEASES(2017)
摘要
The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.
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