Association Of Single Nucleotide Polymorphisms And Haplotypes Of Interleukin-10 5 ' Flanking Region With Systemic Lupus Erythematosus Susceptibility In Han Chinese

Objective: To investigate the association of three SNP at -1082, -819, -592 and haplotypes of IL-10 5'flanking region with systemic lupus erythematosus susceptibility in Han population. Methods: 579 Han Chinese SLE patients fulfilling the ACR criteria and 632 ethnically matched controls were genotyped for IL-10 -1082 G>A, -819 C>T and -592C>A by TaqMan assay. Haplotypes were reconstructed via a mathematical model and analyzed by SHEsis software. Results: IL-10-1082G/A was associated with the susceptibility to SLE (OR=2.858, 95% CI: 2.245-3.640, P<0.001; GG vs. AA, OR=5.623, 95% CI: 2.299-13.754, P<0.001; GA vs. AA, OR=3.147, 95% CI, 2.324-4.261, P<0.001; GG+GA vs. AA, OR=3.313, 95% CI, 2.472-4.440, P<2=22.936, P chi 0.001). No significant differences were observed between SLE patients and controls in IL-10 promoter at position -819 C/T and -592 C/A. Analysis of the haplotypes revealed the haplotype ATA (formed by -1082A, -819T and -592A) that appeared to be a significantly protective effect against Chinese SLE patients (OR=0.663, 95% CI: 0.560-0.785, <2=3.736, P=0.053). chi(2)=3.752, P=0.053; GCC, OR=1.311, 95% CI: 0.996-1.727, chi 0.001). The ACC and GCC appeared no significance between patients and controls (ACC, OR=0.824, 95% CI: 0.677-1.002). Conclusion: Our findings strongly suggested that -1082G/A polymorphism of IL-10 promoter was associated with SLE and ATA haplotype may play a protective role in SLE of Chinese Han population.