Analysis Of The Origin Of Citrus Changshan-Huyou By Dna Barcode

Citrus changshan-huyou Y. B. Chang is a high quality fruit with a therapeutic value. It is produced in the Changshan County of the Zhejiang Province in China, but to date its origin remains unclear. In this work, we studied six plant samples, including the "ancient plant" of C. changshan-huyou, the Cuihong cultivar of C. changshan-huyou, sweet orange (Citrus sinensis Osbeck), mandarin orange (Citrus reticulata Blanco), pomelo (Cifrus maxima) and ponkan (Cifrus ponki Hort). Specifically, we employed seven pairs of primers generally used in identifying species, including the characteristic gene fragments of chlorophyll (rbcL, rpoC1, rpoB, and trnH-psbA), ribosome (5.8S-28S rRNA/ITS1; 18S-5S rRNA/ITS2), and mitochondria (nad1 exonB-C). The phylogenetic tree of C. changshan-huyou was constructed after PCR, cloning, and sequencing of the six plant samples. It was found that (1) pomelo was a female parent of C. changshan-huyou based on the results of ITS2, rbcL, rpoC1, and rpoB; (2) sweet orange was a either a male or a female parent of C. changshan-huyou based on the results of ITS1, rpoC1, trnH-psbA, and nad1 exonB-C, and (3) ponkan was a male parent of C. changshan-huyou based on the results of ITS1 and nadl exonB-C. From the results, it could be argued that the origin of C. changshan-huyou involved the hybridization of multiple sources, including pomelo, sweet orange and ponkan. (C) 2019 Friends Science Publishers