Enhanced Xylanase Production From Thermomyces Lanuginosus Ncim 1374/Dsm 28966 Using Statistical Analysis

Extracellular xylanase production by Thermomyces lanuginosus (NCIM-1374/DSM 28966) could be enhanced using statistical tools of Response surface methodology. Optimized process parameters (pH: 6.3; temperature: 52 degrees C; fermentation time: 108 h; substrate concentration: 1.8% wheat bran) were obtained using ANNOVA. Saccharification process under optimized condition enhanced extracellular xylanase production from 10012 IU/L/min to 12060 IU/L/min. Experimental design showed high correlation between predicted and experiment R-squared values, and analysis of variance had computed F-value of 16.57 with a very low P-value indicating the statistical significance of the quadratic model that can be used to navigate the design space with an adequate precision measure and could optimize the process for high level xylanase production. Thermomyces lanuginosus NCIM1374/ DSM 28966 is an indigenous strain isolated in our laboratory and its xylanase has unique capacity of hydrolyzing xylan to xylose. In the present study we were able to enhance extracellular production of this swift and differently acting enzyme.