Biological Properties Of Human Adipose-Derived Stem Cells Collagen Sponge Complex In Promoting Angiogenesis

This study aims to explore the clinical biological properties of human adipose-derived stem cells-collagen sponge complex (ADSCs-CSC) in promoting angiogenesis. The biological properties of ADSCs were determined by flow cytometry, and the porosity and adhesive rate of ADSCs were detected. The proliferation rate of ADSCs in ADSCs-CSC was determined by Cell Counting Kit (CCK) assay. The tubule formation of HUVECs was detected by Matrigel assay. Vascular endothelial growth factor (VEGF) expression was determined by ELISA assay and RT-qPCR assay. The counts of newborn vessels were determined with chorioallantoic membrane (CAM) assay. The surface signs of the P3 generation of ADSCs were CD34-negative and CD90-positive; meanwhile, the lipid droplet secretion and alizarinred assay proved the successful culture of ADSCs. The adhesive rate of ADSCs-CSC reached to 93.04 +/- 0.67%. The proliferation and tubule formation of HUVECs were notably enhanced, and content and transcription level of VEGF in ADSCs-CSC group were improved significantly than those by ADSCs alone. The counts of newborn vessels were enhanced by nearly twice in ADSCs-CSC implanted on CAM than that in ADSCs implanted alone. ADSCs could adhere to medical-grade collagen sponge with good biocompatibilities, and further promote angiogenesis by enhancing the proliferation of ADSCs and the secretion of VEGF.