In Vitro Plant Regeneration And Hairy Roots Induction In Dioscorea Alata (Cultivar Wenshanyao)

Wenshanyao is a special cultivar of yam (Dioscorea alata L.) cultured in Zhejiang Province in China with both culinary and medicinal values. In this study, the stem segments with leaf axils of yam cv. Wenshanyao were used as explants to induce callus in MS medium with 2 mg/L of 2,4-D. The induction rate reached up to 95%. The callus was cultured in MS+6-BA 2 mg/L+IAA 0.5 mg/L medium and differentiated into clustered buds with a differentiation rate of 86.7%. Buds were cultured in MS+1.0 mg/L IAA medium and regenerated whole plants, in which each root system had 1-4 or more roots. Agrobacterium rhizogenes K599 (with recombinant plasmid pRI101-AN-gfp) was used to infect the seedling stems of Wenshanyao and to obtain hairy roots, which were identified as transgenic hairy roots by PCR and fluorescence microscopy. The tissue-culture regenerated plants obtained in this study provided foundation for the genetic improvement of Wenshanyao varieties. The technology for obtaining transgenic hairy roots provided technical support for the industrialized production of medicinal ingredient of Wenshanyao using hairy root cultures. (C) 2019 Friends Science Publishers