Knockdown of adiponectin promotes the adipogenesis of goat intramuscular preadipocytes

Intramuscular fat (IMF) content determined by the intramuscular preadipocytes differentiation has a huge influence on the sensory quality traits of meats. It was reported that the adiponectin (ADIPOQ) gene could promote adipocytes differentiation, but the underlying molecular and functional characterization of theADIPOQfor regulating goat IMF deposition remained unknown. Herein, the knockdown ofADIPOQwas mediated by siRNAs during goat intramuscular preadipocytes differentiation. Also, the qRT-PCR technique was performed to detect the mRNA levels of target genes in multiply experiment groups. These results showed that theADIPOQwas expressed more than similar to 400 folds in subcutaneous adipose tissue compared to that of heart tissue, and the mRNA level ofADIPOQreached a peak at Hour 60 during the differentiation process, while at Hour 36 didADIPOR1 andADIPOR2. Moreover, the knockdown ofADIPOQpromoted the intramuscular preadipocytes differentiation and accelerated the lipid accumulation in the mature adipocytes with down-regulating theADIPOR1 and preadipocyte factor 1 (Pref-1) mRNA levels and up-regulating the mRNA expression levels of the CAAT/enhancer-binding proteins (C/EBPs) and transcription factor peroxisomal proliferator-activated receptor gamma (PPAR gamma), etc. Our study will provide a new opposite insight that the inhibition ofADIPOQexpression during intramuscular preadipocytes differentiation promotes goat IMF deposition.