MP25-01 THE URINARY MICROBIOME OF HEALTHY ASYMPTOMATIC MEN AND WOMEN: A REFERENCE FOR INTERPRETATION OF NEXT GENERATION SEQUENCING URINE ANALYSES IN SYMPTOMATIC PATIENTS

You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder I (MP25)1 Sep 2021MP25-01 THE URINARY MICROBIOME OF HEALTHY ASYMPTOMATIC MEN AND WOMEN: A REFERENCE FOR INTERPRETATION OF NEXT GENERATION SEQUENCING URINE ANALYSES IN SYMPTOMATIC PATIENTS J. Curtis Nickel, Caleb D. Phillips, Craig D. Tipton, Whitney Stanton, Niccole Diaz, Rick Martin, and E. David Crawford J. Curtis NickelJ. Curtis Nickel More articles by this author , Caleb D. PhillipsCaleb D. Phillips More articles by this author , Craig D. TiptonCraig D. Tipton More articles by this author , Whitney StantonWhitney Stanton More articles by this author , Niccole DiazNiccole Diaz More articles by this author , Rick MartinRick Martin More articles by this author , and E. David CrawfordE. David Crawford More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000002022.01AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The rapid evolution of non-culture technology, such as next generation sequencing (NGS), has led to a surge of patients (and their physicians) submitting midstream urine specimens for NGS analyses. The challenge has been to interpret the comprehensive microbiota data generated by this state-of-the-art technology. We sought to describe the normal urinary microbiome of healthy men and women without urinary disease so that we can better utilize NGS microbiome reports of patients with symptoms. METHODS: Asymptomatic subjects with no recent UTI, antibiotic use within 2 weeks or history of bladder pain were invited to provide demographic data and midstream urine specimen for urinalysis (excluded if positive) and NGS analysis (MicroGenDX). Independent and interaction effects of sample factors (e.g. gender, age, diet, menopausal status) on microbiome composition, abundance and diversity was assessed. RESULTS: NGS data was available for 106 female and 105 male subjects (median age 39). The taxonomic classification of observed bacterial lineages included 643 species (307 genera) with largest number of species detected in Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes phylum (Figure). Gender and antibiotic use within the past 3 months were associated with differential alpha (within sample) diversity. However, the relationship between alpha diversity and age was not significant. Compositional differences in microbiome among samples (beta diversity) was explained by gender and age with gender being the most influential. Gender was also identified as the variable that significantly explained differences in species abundance, while diet and menopausal status were not significant factors. Considering both genders led to identification of two community state types (CST1,2), with most males included in CST1 while females were classified into both. Further investigation within gender suggested 3 female CSTs, one of which was comprised of significantly younger participants. CONCLUSIONS: Interpretation of NGS analyses of urine specimens for possible bacterial infection as a cause for bladder symptoms and pain will need to consider the differential impact of gender, and to a lesser extent, age, and previous antibiotic use. Source of Funding: MicroGenDX © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e453-e453 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information J. Curtis Nickel More articles by this author Caleb D. Phillips More articles by this author Craig D. Tipton More articles by this author Whitney Stanton More articles by this author Niccole Diaz More articles by this author Rick Martin More articles by this author E. David Crawford More articles by this author Expand All Advertisement PDF downloadLoading ...