Activity of Genes and Noncoding RNAs as an Approach to Determination of Early Biomarkers of Radiation-Induced Cancer in Mice

The activity of genes and noncoding RNAs was studied in 183 hybrid mice (CBA × C57Bl) F 1 after a prolonged low-power irradiation (within 21 h at a dose 12.6 Gy with a dose rate 10 mGy/min). The analysis of the studied parameters was carried out in the bone marrow of mice in the eighth and tenth months after irradiation. After ten month, radiation-induced malignant lymphomas in the liver, abdominal cavity, and subcutaneously were found in 14 out of 94 animals. The studied molecular genetic parameters were divided into two groups: oncogenes and tumor suppressors, the activation of which was compared at eighth and tenth months after irradiation. It was detected that the activity of tumor suppressors ( PTEN gene and lncp21 long noncoding RNA) in the groups of mice with tumors was lower in comparison with the group of mice without cancer, while the activity of oncogenes ( NFkB ( p65 ), IkB α, iNOS , TAL1 , CTCF , NEAT1 lncRNAs, and miR-125b) increased. The studied parameters can be considered as potential biomarkers of radiation-induced cancer.