Assessment Of Bone Regeneration Using Tooth Ash And Injectable Platelet Rich Fibrin: A Microcomputed Tomographic (Ct) Analysis

Introduction: One of the main challenge of bone graft or socket preservation in particular is to get good quality and quantity of bone in short time prior to implant bed preparation. The buccal bone at the crest of the ridge is a very thin bone and usually resorb faster than the rest of alveolar bone which may hinder an optimum dento-osseous implant insertion. The purpose of this study will be to assess the bone regeneration capabilities of Tooth Ash Particles (TAP) with injectable Plasma Rich Fibrin (i-PRF) and Tooth Ash Particles (TAP) alone at defects created in the goat mandible bone using micro-computed tomographic (micro-CT). Materials and Methods: A total, 54 bone defect (5 mm x 8 mm) were performed in the 18 goats. The created defect received different treatment (Tx): Tx.A: Unfilled defect (allow natural bone regeneration; Tx.B: Tooth Ash particle (TAP) alone: Tx.C: Tooth Ash + injectable PRF (TAP/i-PRF). Six goats, were sacrificed at different time points:Group 1: at 2nd week, Group2: at 5th week and Group3 at 8th week. The newly formed bone (NFB) was analyzed using micro-CT at different time points. Quantitative and qualitative assessment were carried out namely; the volume of new bone formation (NF-BV) within the defect and its mineral density (NF-BMD), Trabecular Thickness (Tb Th), Trabecular Number (Tb N) and Trabecular Separation (Tb Sp). Result: By 8th week, the mean NF-BV was 69.4821 6.554 mm(3) (cubic millimeter), 65.872 +/- 6.804 mm(3), 26.820 +/- 14.643 mm(3), while the mean NF-BMD was 0.417 +/- 0.119 g/mm(3), 0.786 +/- 0.036 g/mm(3), 0.805 +/- 0.033 g/mm 3 for the defects which received Tx.C, Tx.B and Tx.A respectively. At 8th week Tb Th of NFB was 0.612 +/- 0.168, 0.913 +/- 0.112, and 0.701 +/- 0.126, Trabecular Number of NFB was 2.062 +/- 0.946, 1.002 +/- 0.155, and 1.816 +/- 2.042 and, Trabecular Separation of NFB was 0.330 +/- 0.131, 0.559 +/- 0.110, and 0.495 +/- 0.258 for the defects which received Tx.A, Tx.B and Tx.C respectively. Conclusion: Micro-CT study demonstrated that tooth ash particles mixed with injectable Platelet Rich Fibrin (i-PRF) on mandibular bone defect in goat's model, resulting in new bone with significantly higher volume, mineral density and less remodeling rate when compared with normal bone regeneration of unfilled defects.