Enzymes activity of microprapagated antigonon leptopus plant under effect of salinity stress

The micropropagation ability of Antigonon leptopus was investigated under different concentrations of NaCl (0, 1, 2, 4 and 8 g/L). Two levels of Iron Oxid Nanoparticles (5 and 10 mg/L) was examined to mitigate the effect of salt stress on micropropagated plants. The results showed that NaCl at different concentrations significantly reduced in vitro recorded shoots and roots parameters (survival%, number of shootlets, shootlet length, number of leaves, rooting%, number and length of roots). Moreover, proline content and enzymes (GSH, SOD and Catalase) were increased with increasing NaCl concentration in the culture medium. In addition, adding Iron Oxide NPs to the culture medium showed positive effect for alleviating salt stress on the micropropagated plants. Undertaking there are no reports on the in vitro growth behavior of this important medical plant under salinity stress. So, the aim of this study was to evaluate the adverse effect of salt stress on Antigonon leptopus plants and the possibility of using of Iron oxide nanoparticles to overcome these effects.