Mechanism of Wnt Pathway on Cartilage Differentiation of Adipose Derived Stem

Objective Exploring the interaction of Wnt signaling pathway and sox9 to elucidate the role of adipose-derived stem cells in chondrogenic differentiation. Methohs Through LiCl,DKK-1 stimulate ADSCs into cartilage differentiation, detection of middle-late late early differentiation into cartilage indicators such as collagen type II (Collagen2a), gather the proteoglycan (Aggrecan) and Wnt pathways key protein Sox9, BMP-2, beta serial protein (beta-catenin), glycogen synthase kinase 3 beta (GSK-3 beta) expression.Subsequently, lentivirus was used as the vector, the full-length Sox9 gene was transfected into ADSCs for stable expression, and the expression levels of relevant indicators were detected again. Results During the chondrogenic induction and differentiation of ADSCs, the Wnt pathway promoted the rapid proliferation of ADSCs in the early, middle and late stages and induced the expression of Collagen 2a Aggrecan to increase in the early stage but not significantly (P<0.05).At the later stage, the phenotype of mature cartilage was inhibited and cartilage hypertrophy and early cartilage ossification were promoted.Overexpression was fed back to inhibit the expression of Sox9, the activity of Wnt pathway, up-regulated in early, mid-stage and down-regulated in late stage (P<0.01). Conclusion The Wnt pathway regulates chondrogenic differentiation of ADSCs by regulating Sox9, but its regulatory mechanism is significantly different at different stages of induced differentiation.