茶树炭疽病拮抗内生细菌贝莱斯芽胞杆菌的筛选与鉴定

DAI Yafeng,WU Nannan,GAO Zhen,WANG Gaozhan,GONG Andong,ZHANG Yimei

wf（2021）

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Abstract

从祥云茶业基地信阳10号母本园中采摘信阳10号茶树(Camellia sinensis)健康叶片,分离纯化得到茶树内生细菌7株,平板对峙法筛选得到4株拮抗菌,均对茶树炭疽菌(Colletotrichum fructicola)具有抑制作用,其中X10-03抑菌效果最好,其代谢产物对茶树炭疽病病菌的抑菌率达到52.7％.光学显微镜观察发现,X10-03菌株代谢产物可有效抑制茶树炭疽菌分生孢子萌发和菌丝的生长.稀释3倍的发酵液对茶树炭疽菌菌丝生长的抑菌率达到70.4％;经过16S rDNA和gyrA基因序列分析,将内生拮抗细菌X10-03鉴定为贝莱斯芽胞杆菌(Bacillus velezensis).

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