Sensitive electrochemiluminescence analysis of lung cancer marker miRNA-21 based on RAFT signal amplification

An electrochemiluminescence approach based on surface-initiated reversible addition–fragmentation chain transfer (SI-RAFT) was developed for miRNA-21 detection for the first time. The SI-RAFT polymerization generates polymer chains with functional groups that are used to recruit luminol, enabling strong ECL signal output with low concentrations of miRNA-21, and greatly improving the detection sensitivity.