Effect of Kaempferol and Its Glycoside Derivatives on Antioxidant Status of HL-60 Cells Treated with Etoposide

Kaempferol is a well-known antioxidant found in many plants and plant-based foods. In plants, kaempferol is present mainly in the form of glycoside derivatives. In this work, we focused on determining the effect of kaempferol and its glycoside derivatives on the expression level of genes related to the reduction of oxidative stress-NFE2L2, NQO1, SOD1, SOD2, and HO-1; the enzymatic activity of superoxide dismutases; and the level of glutathione. We used HL-60 acute promyelocytic leukemia cells, which were incubated with the anticancer drug etoposide and kaempferol or one of its three glycoside derivatives isolated from the aerial parts of Lens culinaris Medik.-kaempferol 3-O-[(6-O-E-caffeoyl)-beta-d-glucopyranosyl-(1 -> 2)]-beta-d-galactopyranoside-7-O-beta-d-glucuropyranoside (P2), kaempferol 3-O-[(6-O-E-p-coumaroyl)-beta-d-glucopyranosyl-(1 -> 2)]-beta-d-galactopyranoside-7-O-beta-d-glucuropyranoside (P5), and kaempferol 3-O-[(6-O-E-feruloyl)-beta-d-glucopyranosyl-(1 -> 2)]-beta-d-galactopyranoside-7-O-beta-d-glucuropyranoside (P7). We showed that none of the tested compounds affected NFE2L2 gene expression. Co-incubation with etoposide (1 mu M) and kaempferol (10 and 50 mu g/mL) leads to an increase in the expression of the HO-1 (9.49 and 9.33-fold at 10 mu g/mL and 50 mu g/mL, respectively), SOD1 (1.68-fold at 10 mu g/mL), SOD2 (1.72-fold at 10-50 mu g/mL), and NQO1 (1.84-fold at 50 mu g/mL) genes in comparison to cells treated only with etoposide. The effect of kaempferol derivatives on gene expression differs depending on the derivative. All tested polyphenols increased the SOD activity in cells co-incubated with etoposide. We observed that the co-incubation of HL-60 cells with etoposide and kaempferol or derivative P7 increases the level of total glutathione in these cells. Taken together, our observations suggest that the antioxidant activity of kaempferol is related to the activation of antioxidant genes and proteins. Moreover, we observed that glycoside derivatives can have a different effect on the antioxidant cellular systems than kaempferol.