The Use of Steam Pretreatment to Enhance Pellet Durability and the Enzyme-Mediated Hydrolysis of Pellets to Fermentable Sugars.

Although densified wood pellets are an attractive biomass feedstock for bioenergy and biofuels production, partly due to their ease of transport, their friability and hygroscopic nature (attraction of moisture) have proven problematic in terms of storage and handling. Pre-steaming the biomass was shown to reduce the need for size reduction, significantly increasing pellet durability by relocating the plant cell wall lignin to the fibre surface and consequently enhancing binding between particles. Although steam pretreatment has been shown to facilitate enzyme-mediated hydrolysis of biomass, by increasing cellulose accessibility, drying and pelletization partially impeded enzymatic hydrolysis. However, the incorporation of alkaline deacetylation or neutral sulfonation step prior to pre-steaming was shown to mitigate many of the negative effects of drying. Although drying and pelletization did not significantly impact the redistribution of lignin, a mild mechanical refining step was shown to further enhance the hydrolysis of the cellulose component of the pelletized biomass.