Effect of ion-pairing reagent hydrophobicity on liquid chromatography and mass spectrometry analysis of oligonucleotides

Journal of Chromatography A(2022)

引用 21|浏览5
暂无评分
摘要
•We proposed an RP LC method for the classification of ion-pairing reagents.•Hydrophobic (stronger) alkylamine ion-pairing reagents give greater separation of oligonucleotides.•Ion-pairing RP LC of oligonucleotides behaves as a mixed mode chromatography.•The separation selectivity depends on the pairing reagent hydrophobicity.
更多
查看译文
关键词
Ion-pairing,Therapeutic oligonucleotides,Alkylamine,Peak capacity,Reversed-phase
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络