Quantitation of Human 14-3-3ζ Dimerization and the Effect of Phosphorylation on Dimer-monomer Equilibria

Zuzana Trošanová,Petr Louša,Aneta Kozeleková,Tomáš Brom,Norbert Gašparik,Ján Tungli,Veronika Weisová,Erik Župa,Gabriel Žoldák,Jozef Hritz

Journal of Molecular Biology（2022）

引用 8|浏览7

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摘要

•Quantitative analysis of homo and heterodimerization of 14-3-3 proteins.•Novel fluorescence assays to determine KD and kinetics of 14-3-3 dimerization.•Phosphorylation of S58 increases dimerization KD of 14-3-3 from nM to mM range.•Phosphorylated clients and 14-3-3 protein antagonize each other.•Conceptual framework for the quantification of dimeric protein families.

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关键词

14-3-3,phosphorylation,dimerization,dissociation constant,FRET,NMR

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