Receptor-mediated Endocytosis is an Important Way for Gelatin Nano-Particles Penetration into Cells

Experimental data detailing the possibilities of using gelatin nanoparticles obtained by the two-stage desolvation method without the use of surfactants for delivering pharmacologically active substances to cultured normal and human cancer cells are presented. It was shown that clathrin-dependent endocytosis is the main route of entry of such nanoparticles into normal human fibroblasts and cancer cells of the MDA-MB-231 line. Due to this type of endocytosis, more than 50 % of gelatin nanoparticles enter the cells. It was shown that in the process of clathrin-dependent endocytosis, gelatin nanoparticles specifically bind to collagen receptors.