A Similarity-Based Method for Predicting Enzymatic Functions in Yeast Uncovers a New AMP Hydrolase

•A quarter of the Saccharomyces cerevisiae genome is not functionally annotated.•We created a database of sensitive sequence similarity predictions for yeast proteins.•We use our database to identify candidate enzymes for non-annotated reactions.•We demonstrate a new function for the previously uncharacterized enzyme Yhr202w.•Our methodology allows uncovering new functions of uncharacterized proteins.