Characterisation of the biosynthesised gold nanomaterials - spectroscopic, dimensional, morphological, stability and structural analysis

The biosynthesised gold nanoparticles were characterised using various standard techniques. The preliminary confirmation was carried out by UV-Vis spectrophotometry. Electron microscopy analysis was carried out by SEM, HRSEM and TEM. The elemental analysis was performed through EDAX on SEM and HRSEM. The SAED pattern of the synthesised gold nanoparticles was analysed by TEM. The zeta potential measurements of all the nanoparticles were observed in concordance with the standard reference values demonstrating highly stable nanoformulations. The plant extracts and nanoparticles were analysed by FTIR spectroscopy. X-ray diffraction (XRD) patterns have been analysed for the biosynthesised nanoparticles and nanoconjugates. ICP-OES elemental analysis was carried out to quantify the concentration of elemental gold present in the synthesised gold nanoparticles.