Preparation, characterization, and bioevaluation of Tc-99m-famotidine as a selective radiotracer for peptic ulcer disorder detection in mice

This work focuses on tracking peptic ulcer localized in mice. The formation of a [Tc-99m]dithiocarbamate of famotidine complex at optimum conditions of reaction temperature (37 degrees C), reaction time (30 min), pH of the reaction mixture (5), amount of substrate (100 mu g), amount of reducing agent (tin (II) content, 50 mu g), was achieved using radioactive Tc-(99m) (250-400 MBq), with labelling yield of 98% and high radiochemical purity. The in-vitro stability of [Tc-99m]dithiocarbamate of famotidine complex was shown to be high in rat serum for up to 8 h. Normal and ulcerated mice were used in biodistribution studies. Famotidine works by blocking histamine-2-receptor antagonists (H2RAs). The high absorption of [Tc-99m]dithiocarbamate of famotidine complex in stomach in amount of 27.15% injected dose/g organ (ID/g) observed in ulcerated mice for up to 24 h demonstrated its usefulness as a novel radiotracer for stomach imaging.