Influence of Semen Processing on Proportion of Aneuploid Sperm

Objective:To analyze the influence of semen processing methods on the proportion of the aneuploid sperm by detecting the sperm's chromosome X,Y,18 using fluorescence in situ hybridization.Methods:Ten patients with mild oligoasthenosperia,who were received ICSI treatment,were included in this study.Five semen samples of the patients were randomly selected to detect using Swim-up method(A group) and 5 using sperm-grad double-density centrifugation method(B group).Another 5 patients with mild oligo-asthenosperia were as control(C group).The CEPX /Y and CEP18 probe was used to detect the sperm of these 15 patients by fluorescence in situ hybridization.The proportion of aneuploid sperm was compared in three groups.Results:The sex chromosome aneuploid rates were(4.21±2.49) %,(3.24±1.49) % and(2.62±0.89) % in control,A and B groups.The rates of aneuploid chromosome 18 were(3.00±1.22)%,(2.00±1.22)% and(2.00±1.22)% in control,A and B groups.There were no significant differences in three groups(P 0.05).Conclusion:The results showed that the methods of Swim-up and Sperm-Grad double-density gradient centrifugation could select sperms in motility potential and teratospermia,but not in normal chromosome sperms.