Biodegradable and Injectable Sealant With Photo- Regulated NO Generator for MRSA-Infected Wound Healing

The development of degradable sealant hydrogel with high antibacterial effect and wound-healing capacity is in urgent need for the treatment of open wounds infected by multidrug-resistant (MDR) bacteria. Herein, an injectable, degradable, and photo-activated antibacterial hydrogel (MPDA-BNN6@Gel) has been developed by incorporating BNN6-loaded mesoporous polydopamine nanoparticle (MPDA-BNN6 NPs) into a fibrin-based hydrogel. After the administration of MPDA-BNN6@Gel, the MPDA-BNN6@Gel could generate local hyperthermia and release large quantities of NO gas to treat the Methicillin-resistant Staphylococcus Aureus (MRSA) infection under the stimulation of an 808 nm laser. The experiments confirmed that the bacteria were eradicated through irreversible damage to the cell membrane, energy metabolism, and genetic material. Furthermore, in the absence of laser, the fibrin and a small amount of NO originating from MPDA-BNN6@Gel could promote wound healing in vivo. This work indicates that the MPDA-BNN6@Gel is a promising alternative for the treatment of infected wounds and provides a facile tactic to design a photo-regulated bactericidal hydrogel sealant for accelerating infected wound healing.