Treponema primitia α1–2-fucosyltransferase-catalyzed one-pot multienzyme synthesis of fucosylated oligosaccharide lacto-N-fucopentaose I with antiviral activity against enterovirus 71

•A novel α1,2-fucosyltransferase was cloned from Treponema primiti.•Tpα1,2-FT catalyzes one-pot multi-enzyme synthesis of lacto-N-fucopentaose-I.•LNFP-I was an inhibitor of enterovirus 71 in the early stages of infection.