Monitoring COVID-19 spread in Prague local neighborhoods based on the presence of SARS-CoV-2 RNA in wastewater collected throughout the sewer network

•SARS-CoV-2 RNA presence was measured at 24 locations in the Prague sewer network.•Residential areas (100–13,000 inhab.), transport hubs, hospitals etc. were included.•Consistent wastewater monitoring by RT-mqPCR took place from August 2020–May 2021.•The sampling of major Prague sewers correlated well with clinical-based data.•Grab samples can identify COVID-19 hotspots in local neighborhoods.