Therapeutic Effect of Nile Tilapia Type II Collagen on Rigidity in CD8(+) Cells by Alleviating Inflammation and Rheumatoid Arthritis in Rats by Oral Tolerance

Fibrillins are microfibril-associated macro glycoproteins found in connective tissues and structurally related to latent TGF-beta-binding proteins (LTBPs). The special cellular immunity and blocking glycoprotein receptors IIb and IIIa of fibrillins are emerging topics in recent years. In this study, Nile Tilapia type IIcollagen (NTCII) was extracted and purified from the skull cartilages by a pepsin-soluble method. Amino acid analysis indicated that NTCII consisted of 315/1000 glycine residues, 72/1000 hydroxyproline residues and 108/1000 proline residues. SDS-PAGE analysis showed that NTCII was composed of three identical 130 kDa alpha-chains. The results of glycoprotein/carbohydrate assay indicated that the total polysaccharide content of NTCII was 5.6-19.0%. The IR spectrum of NTCII displayed five characteristic peaks of amide I, II, III, A, B. NTCII at 10-100 mu g/mL concentration downregulated the content of cytokines in the presence or absence of LPS, especially the secretion of cytokines IL-6, IL-1 beta and TNF-alpha. Interestingly, NTCII promoted the secretion of Fas/Apo-1 compared to the control group and 25 mu g/mL of NTCII resulted in a higher Fas/Apo-1 secretion level in CD8(+) T cells. FITC-TCII fluorescence images confirmed that NTCII could bind to the membrane surface of CD8(+) T cells, leading to the induction of rigidity. NTCII could bind to the membrane surface of CD8(+) T cells that leads to the induction of rigidity, as evidenced by the FITC-NTCII fluorescence images. The qRT-PCR gene expression analysis of caspase-8 collected with Fas/Apo-1 was upregulated significantly in the 1 and 50 mu g/mL NTCII-treated groups compared with the control group. Overall, the results conclude that the rigidity did not lead to an increase in inflammatory factors in CD8(+) T cells treated with NTCII. The oral administration of NTCII 3 mg/kg dosage caused more prominent repair of damaged ankle cartilage than the 1 mg/kg dosage in Freund's adjuvant-induced model of arthritis in rats. Therefore, this study disclosed the immunological and anti-arthritic effect of fibrillar collagen, which could be a potential biomaterial for practical applications with lower toxicity.