Allosteric Regulation of the Soluble Epoxide Hydrolase by Nitro Fatty Acids: a Combined Experimental and Computational Approach

•hsEH is a therapeutic target, but no approved drug is available.•hsEH CTD is allosterically regulated by electrophilic fatty acids.•New covalent modification sites were identified as latent allosteric sites.•Distinct electrophilic fatty acids differentially modulate allosteric propagation.•Allosteric inhibition strategies could circumvent limitations of orthosteric drugs.