Mechanistic investigations of hirsutene biosynthesis catalyzed by a chimeric sesquiterpene synthase from Steccherinum ochraceum

Qiwen Wang,Ji-Kai Liu,Qunfei Zhao,Qing-Li He

Fungal Genetics and Biology（2022）

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摘要

•First heterologous expression of chimeric STS A8411 in Aspergillus oryzae.•The absolute configuration of hirsutene was determined by x-ray diffraction.•The Biosynthetic mechanism of hirsutene was elucidated by isotope labeling experiment.

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关键词

Terpene synthases,Cyclization mechanism,Biosynthesis of hirsutene,Isotope labeling experiments,Steccherinum ochraceum

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