In Vitro Quantification of Cancer Stem Cells Using a Mammosphere Formation Assay.

Nick A Kuburich,Petra den Hollander,Abhijeet P Deshmukh,Suhas Vasaikar,Robiya Joseph,Max S Wicha,Sendurai A Mani

Methods in molecular biology (Clifton, N.J.)（2022）

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摘要

Cancer stem cells (CSCs) are a small subpopulation of self-renewing cancer cells that are present within tumors. In this chapter, we provide a detailed method for the quantification of CSCs in vitro through mammosphere formation.

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关键词

Cancer stem cells (CSC),Mammosphere,Stemness,Suspension,Tumorsphere

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