Ep049: Discordant Fluorescence in Situ Hybridization and RNASeq Results in the Identification of Fusion Partners in Recurring Translocations in Hematological Malignancies

Reciprocal chromosomal translocations are implicated in neoplastic transformation in tumors. These can be identified by chromosome analysis, by fluorescence in situ hybridization (FISH) if cryptic, or by RNASeq/next generation sequencing. FISH probes are highly specific and sensitive, and have the advantage of faster turn-around-time, are less expensive, and can be used on a variety of specimen types. Therefore, several of these probes are commercially available, and are incorporated to screen diagnostic specimens.