Investigation of Diazepam Sequential Metabolism with Hepatocytes via a Microfluidic Device.

Generally, this microfluidic device can characterize the sequential metabolism of diazepam. The C-t profiles of DZP and its metabolites are similar to that of the liver single-pass perfusion study. Due to the existence of transporters, the lag time of T-G is similar to that of primary metabolites. As a future study, the distribution of DZP and its metabolites along the device will be measured by MALDI imaging. The information of spatial distribution is expected to further help optimize and validate the mathematical models.