Novel insights on the near atomic scale spatial distributions of substitutional alloying and interstitial impurity elements in Ti-6Al-4V alloy

•Spatial distribution of elements has significant impact on mechanical response.•Characterization of atomic scale distribution of elements in Ti-6Al-4V alloy.•Simultaneous segregation of interstitial impurities within the α phase.•Inverse interrelation between interstitial Oxygen and substitutional Al atoms.•Findings are explained based on the lattice distortion and supported by DFT.