Continuous bioreactor bioprocess using immobilized Spathaspora passalidarum to convert hydrolysates of oat and soybean hulls into ethanol

BIOMASS CONVERSION AND BIOREFINERY(2024)

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Abstract
We investigated the performance of fluidized and packed-bed bioreactors using cells of Spathaspora passalidarum UFMG-CM-469 immobilized on LentiKats (R) in the fermentation of lignocellulosic biomass hydrolysates. Acid (AH-CYE) and enzymatic (EH-CYE) hydrolyses of a 1:1 mixture of oat and soybean hulls, minimally supplemented with crude yeast extract, were used as substrates. Continuous cultures at dilution rate (D) of 0.05 h(-1) were run in the fluidized and packed-bed bioreactors, using both AH-CYE and EH-CYE, showing ethanol yields varying from 0.30 to 0.45 g.g(-1) and productivities from 0.39 and 0.99 g.(L.h)(-1). Continuous cultures in the fluidized bioreactor, which proved to be better than the packed bed, operating at higher dilution rates of 0.1 h(-1) and 0.2 h(-1) increased ethanol productivity, reaching 1.67 g.(L.h)(-1) when using AH-CYE and 2.65 g.(L.h)(-1) with EH-CYE. These results show the possibility of exploring the fermentation of lignocellulosic biomass hydrolysates in continuous cultures of immobilized S. passalidarum UFMG-CM-469 to produce second-generation ethanol under a concept of biorefinery.
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Key words
Second-generation ethanol,Yeasts from Brazilian biodiversity,Acid and enzymatic biomass hydrolysates,LentiKats (R)
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