Proteomic Analysis of Grain Protein in the Winter Wheat Jimai44 and Jimai229 Based on Strong Gluten Characteristics

Abstract Background：To determine the grain proteome of the strong gluten wheat, TMT technology was used to analyze proteomic differences between the winter wheat varieties Jimai 44 and the strong gluten Jimai 229. Results：Comparisons were also made with the winter wheat Jimai 22 as a control. It was found that there were 120 differentially expressed proteins between Jimai 44 and Jimai 22, while 173 between Jimai 229 and Jimai 22. Among these, 27 proteins were up-regulated and 23 down-regulated in jimai 44 and in jimai 229. Conclusions：GO analysis and KEGG pathway analysis showed that the differentially expressed proteins were mainly enriched in cell functions relating to nutrient reservoir activity, metabolic processes, protein processing in endoplasmic reticulum, phenylpropane biosynthesis and nitrogen metabolism. Amongst the screened proteins, many differences were related to storage proteins. This study provides an important basis for further exploring the key factors of quality improvement and genetic variation of gluten protein expression.