Assessment of fluorescent protein candidates for multi-color flow cytometry analysis of Saccharomyces cerevisiae

Raquel Perruca-Foncillas, Johan Davidsson,Magnus Carlquist,Marie F Gorwa-Grauslund

Biotechnology Reports（2022）

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摘要

•In vivo fluorescence of candidate fluorescent proteins was assessed in Saccharomyces cerevisiae.•eEGFP, CyOFP1opt and mBeRFPopt were found to be suitable for multicolour flow cytometry.•Successful protein selection and equipment configuration allowed potential tri-color flow cytometry with 488 nm single-laser excitation.

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关键词

Fluorescent proteins,Multiple biosensors,Flow cytometry,Biosensors,Population heterogeneity,Saccharomyces cerevisiae

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