In Utero Electroporation and Cranial Window Implantation for in Vivo Wide-Field Two-Photon Calcium Imaging Using G-CaMP9a Transgenic Mice
STAR protocols(2022)
摘要
We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice.For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2022).
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关键词
Developmental biology,Microscopy,Model Organisms,Neuroscience
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